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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Amino acid depletion activates TonEBP and sodium-coupled inositol transport

Cited 21 times inthomson ciCited 22 times inthomson ci
Title
Amino acid depletion activates TonEBP and sodium-coupled inositol transport
Author
Franchi-Gazzola, RVisigalli, RDall'Asta, VSala, RWoo, SKKwon, H. MooGazzola, GCBussolati, O
Keywords
Cell volume; Glutamine; Hypertonic stress; Stress proteins; System A; Tonicity-responsive enhancer-binding protein
Issue Date
200106
Publisher
AMER PHYSIOLOGICAL SOC
Citation
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.280, no.6, pp.C1465 - C1474
Abstract
The expression of the osmosensitive sodium/myo-inositol cotransporter (SMIT) is regulated by multiple tonicity-responsive enhancers (TonEs) in the 5'-flanking region of the gene. In response to hypertonicity, the nuclear abundance of the transcription factor TonE-binding protein (TonEBP) is increased, and the transcription of the SMIT gene is induced. Transport system A for neutral amino acids, another osmosensitive mechanism, is progressively stimulated if amino acid substrates are not present in the extracellular compartment. Under this condition, as in hypertonicity, cells shrink and mitogen-activated protein kinases are activated. We demonstrate here that a clear-cut nuclear redistribution of TonEBP, followed by SMIT expression increase and inositol transport activation, is observed after incubation of cultured human fibroblasts in Earle's balanced salts (EBSS), an isotonic, amino acid-free saline. EBSS-induced SMIT stimulation is prevented by substrates of system A, although these compounds do not compete with inositol for transport through SMIT. We conclude that the incubation in isotonic, amino acid-free saline triggers an osmotic stimulus and elicits TonEBP-dependent responses like hypertonic treatment.
URI
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ISSN
0363-6143
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