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DC Field | Value | Language |
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dc.citation.endPage | 9735 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 9727 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 82 | - |
dc.contributor.author | Peng, Zhiyong | - |
dc.contributor.author | Soper, Steven A. | - |
dc.contributor.author | Pingle, Maneesh R. | - |
dc.contributor.author | Barany, Francis | - |
dc.contributor.author | Davis, Lloyd M. | - |
dc.date.accessioned | 2023-12-22T06:39:28Z | - |
dc.date.available | 2023-12-22T06:39:28Z | - |
dc.date.created | 2013-06-20 | - |
dc.date.issued | 2010-12 | - |
dc.description.abstract | Detection of pathogenic bacteria and viruses require strategies that can signal the presence of these targets in near real-time due to the potential threats created by rapid dissemination into water and/or food supplies In this paper, we report an innovative strategy that can rapidly detect bacterial pathogens using reporter sequences found in their genome without requiring polymerase chain reaction (PCR) A pair of strain-specific primers was designed based on the 168 rRNA gene and were end-labeled with a donor (Cy5) or acceptor (Cy5 5) dye In the presence of the target bacterium, the primers were joined using a ligase detection reaction (LDR) only when the primers were completely complementary to the target sequence to form a reverse molecular beacon (rMB), thus bringing Cy5 (donor) and Cy5 5 (acceptor) into close proximity to allow fluorescence resonance energy transfer (FRET) to occur These rMBs were subsequently analyzed using single molecule detection of the FRET pairs (single-pair FRET, spFRET) The LDR was performed using a continuous flow thermal cycling process configured in a cyclic olefin copolymer (COC) microfluidic device using either 2 or 20 thermal cycles Single molecule photon bursts from the resulting rMBs were detected on chip and registered using a simple laser-induced fluorescence (LIF) instrument. The spFRET signatures from the target pathogens were reported in as little as 2 6 mm using spFRET. | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.82, no.23, pp.9727 - 9735 | - |
dc.identifier.doi | 10.1021/ac101843n | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.scopusid | 2-s2.0-78649701493 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4489 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78649701493 | - |
dc.identifier.wosid | 000284668600021 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Ligase Detection Reaction Generation of Reverse Molecular Beacons for Near Real-Time Analysis of Bacterial Pathogens Using Single-Pair Fluorescence Resonance Energy Transfer and a Cyclic Olefin Copolymer Microfluidic Chip | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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