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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens

Cited 1 times inthomson ciCited 0 times inthomson ci
Title
Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens
Author
Kim, Tae-HyeongPark, JuheeKim, Chi-JuCho, Yoon-Kyoung
Keywords
REAL-TIME PCR; RECOMBINASE POLYMERASE AMPLIFICATION; CENTRIFUGAL MICROFLUIDIC PLATFORMS; ESCHERICHIA-COLI O157; IMMUNOMAGNETIC SEPARATION; RAPID DETECTION; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; DNA EXTRACTION; WHOLE-BLOOD
Issue Date
201404
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.86, no.8, pp.3841 - 3848
Abstract
This paper describes a micro total analysis system for molecular analysis of Salmonella, a major food-borne pathogen. We developed a centrifugal microfluidic device, which integrated the three main steps of pathogen detection, DNA extraction, isothermal recombinase polymerase amplification (RPA), and detection, onto a single disc. A single laser diode was utilized for wireless control of valve actuation, cell lysis, and noncontact heating in the isothermal amplification step, thereby yielding a compact and miniaturized system. To achieve high detection sensitivity, rare cells in large volumes of phosphate-buffered saline (PBS) and milk samples were enriched before loading onto the disc by using antibody-coated magnetic beads. The entire procedure, from DNA extraction through to detection, was completed within 30 min in a fully automated fashion. The final detection was carried out using lateral flow strips by direct visual observation; detection limit was 10 cfu/mL and 10 2 cfu/mL in PBS and milk, respectively. Our device allows rapid molecular diagnostic analysis and does not require specially trained personnel or expensive equipment. Thus, we expect that it would have an array of potential applications, including in the detection of food-borne pathogens, environmental monitoring, and molecular diagnostics in resource-limited settings.
URI
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DOI
http://dx.doi.org/10.1021/ac403971h
ISSN
0003-2700
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