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Park, Su-Moon
Electrochemistry Lab
Research Interests
  • Electrochemistry and spectroscopy of conducting polymers

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Label-Free Impedimetric Sensor for a Ribonucleic Acid Oligomer Specific to Hepatitis C Virus at a Self-Assembled Monolayer-Covered Electrode

Cited 20 times inthomson ciCited 14 times inthomson ci
Title
Label-Free Impedimetric Sensor for a Ribonucleic Acid Oligomer Specific to Hepatitis C Virus at a Self-Assembled Monolayer-Covered Electrode
Author
Park, Jin-YoungLee, Yoon-sukChang, Byoung-YongKim, Byeang HyeanJeon, SangminPark, Su-Moon
Keywords
18-crown-6; Ammonium ions; Before and after; Charge transfer resistance; Covered electrodes; Detection limits; Electrochemical impedance; HCV RNA; Hepatitis C virus; Host guest interactions; Internal ribosome entry site; Label free; Peptide nucleic acid; Rat livers; Redox probe; Ribonucleic acid; Two step method
Issue Date
201010
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.82, no.19, pp.8342 - 8348
Abstract
A ribonucleic acid (RNA) sensor based on hybridization of its peptide nucleic acid (PNA) molecule with a target RNA oligomer of the internal ribosome entry site sequence specific to the hepatitis C virus (HCV) and the electrochemical impedance detection is described. This RNA is one of the most conservative molecules of the whole HCV RNA genome. The ammonium ion terminated PNA molecule was immobilized via its host-guest interactions with the diaza crown ring of 3-thiophene-acetamide-diaza-18-crown-6 synthesized by a simple two-step method, which forms a well-defined self-assembled monolayer (SAM) on gold. Hybridization events of the probe PNA with the target RNA were monitored by measuring charge-transfer resistances for the Fe(CN)(6)(3-/4-) redox probe using Fourier transform electrochemical impedance spectroscopy. The ratio of the resistances of the SAM-covered electrode measured before and after hybridization increased linearly with log[RNA] in the rat liver lysate with a detection limit of about 23 pM.
URI
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DOI
http://dx.doi.org/10.1021/ac1019232
ISSN
0003-2700
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