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Author

Kim, Hajin
Single Molecule Biophysics Lab
Research Interests
  • Biophysics, Single Molecule, fluorescence Spectroscopy, biological Motors, biomolecular assembly

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Protein-guided RNA dynamics during early ribosome assembly

Cited 5 times inthomson ciCited 3 times inthomson ci
Title
Protein-guided RNA dynamics during early ribosome assembly
Author
Kim, HajinAbeysirigunawarden, Sanjaya C.Chen, KeMayerle, MeganRagunathan, KaushikLuthey-Schulten, ZaidaHa, TaekjipWoodson, Sarah A.
Keywords
RNA; RNA folding; Single-molecule biophysics
Issue Date
201402
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE, v.506, no.7488, pp.334 - 338
Abstract
The assembly of 30S ribosomes requires the precise addition of 20 proteins to the 16S ribosomal RNA. How early binding proteins change the ribosomal RNA structure so that later proteins may join the complex is poorly understood. Here we use single-molecule fluorescence resonance energy transfer (FRET) to observe real-time encounters between Escherichia coli ribosomal protein S4 and the 16S 5′ domain RNA at an early stage of 30S assembly. Dynamic initial S4-RNA complexes pass through a stable non-native intermediate before converting to the native complex, showing that non-native structures can offer a low free-energy path to protein-RNA recognition. Three-colour FRET and molecular dynamics simulations reveal how S4 changes the frequency and direction of RNA helix motions, guiding a conformational switch that enforces the hierarchy of protein addition. These protein-guided dynamics offer an alternative explanation for induced fit in RNA-protein complexes.
URI
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DOI
http://dx.doi.org/10.1038/nature13039
ISSN
0028-0836
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