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dc.citation.endPage 214 -
dc.citation.number 2 -
dc.citation.startPage 208 -
dc.citation.title JALA -
dc.citation.volume 19 -
dc.contributor.author Cavnar, Stephen P. -
dc.contributor.author Salomonsson, Emma -
dc.contributor.author Luker, Kathryn E. -
dc.contributor.author Luker, Gary D. -
dc.contributor.author Takayama, Shuichi -
dc.date.accessioned 2023-12-22T02:44:53Z -
dc.date.available 2023-12-22T02:44:53Z -
dc.date.created 2014-03-28 -
dc.date.issued 2014-04 -
dc.description.abstract Three-dimensional culture systems bridge the experimental gap between in vivo and in vitro physiology. However, nonstandardized formation and limited downstream adaptability of 3D cultures have hindered mainstream adoption of these systems for biological applications, especially for low- and moderate-throughput assays commonly used in biomedical research. Here we build on our recent development of a 384-well hanging drop plate for spheroid culture to design a complementary spheroid transfer and imaging (TRIM) plate. The low-aspect ratio wells of the TRIM plate facilitated high-fidelity, user-independent, contact-based collection of hanging drop spheroids. Using the TRIM plate, we demonstrated several downstream analyses, including bulk tissue collection for flow cytometry, high-resolution low working-distance immersion imaging, and timely reagent delivery for enzymatic studies. Low working-distance multiphoton imaging revealed a cell type-dependent, macroscopic spheroid structure. Unlike ovarian cancer spheroids, which formed loose, disk-shaped spheroids, human mammary fibroblasts formed tight, spherical, and nutrient-limited spheroids. Beyond the applications we describe here, we expect the hanging drop spheroid plate and complementary TRIM plate to facilitate analyses of spheroids across the spectrum of throughput, particularly for bulk collection of spheroids and high-content imaging -
dc.identifier.bibliographicCitation JALA, v.19, no.2, pp.208 - 214 -
dc.identifier.doi 10.1177/2211068213504296 -
dc.identifier.issn 2211-0682 -
dc.identifier.scopusid 2-s2.0-84896305360 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/4067 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84896305360 -
dc.identifier.wosid 000332968600010 -
dc.language 영어 -
dc.publisher SAGE PUBLICATIONS INC -
dc.title Transfer, Imaging, and Analysis Plate for Facile Handling of 384 Hanging Drop 3D Tissue Spheroids -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Biochemical Research Methods; Chemistry, Analytical -
dc.relation.journalResearchArea Biochemistry & Molecular Biology; Chemistry -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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