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Lee, Changsoo
Applied Biotechnology Lab for Environment
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dc.citation.endPage 2280 -
dc.citation.number 11 -
dc.citation.startPage 2272 -
dc.citation.title JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA -
dc.citation.volume 25 -
dc.contributor.author Kim, Jaai -
dc.contributor.author Kim, Woong -
dc.contributor.author Lee, Changsoo -
dc.date.accessioned 2023-12-22T03:14:45Z -
dc.date.available 2023-12-22T03:14:45Z -
dc.date.created 2013-11-28 -
dc.date.issued 2013-11 -
dc.description.abstract Anaerobic digestion (AD) is gaining increasing attention due to the ability to covert organic pollutants into energy-rich biogas and, accordingly, growing interest is paid to the microbial ecology of AD systems. Despite extensive efforts, AD microbial ecology is still limitedly understood, especially due to the lack of quantitative information on the structures and dynamics of AD microbial communities. Such knowledge gap is particularly pronounced in sewage sludge AD processes although treating sewage sludge is among the major practical applications of AD. Therefore, we examined the microbial communities in three full-scale sewage sludge digesters using qualitative and quantitative molecular techniques in combination: denaturing gradient gel electrophoresis (DGGE) and real-time polymerase chain reaction (PCR). Eight out of eleven bacterial sequences retrieved from the DGGE analysis were not affiliated to any known species while all eleven archaeal sequences were assigned to known methanogen species. Quantitative real-time PCR analysis revealed that, based on the 16S rRNA gene abundance, the hydrogenotrophic order Methanomicrobiales is the most dominant methanogen group (> 94% of the total methanogen population) in all digesters. This corresponds well to the prevailing occurrence of the DGGE bands related to Methanolinea and Methanospirillum, both belonging to the order Methanomicrobiales, in all sludge samples. It is therefore suggested that hydrogenotrophic methanogens, especially Methanomicrobiales strains, are likely the major players responsible for biogas production in the digesters studied. Our observation is contrary to the conventional understanding that aceticlastic methanogens generally dominate methanogen communities in stable AD environments, suggesting the need for further studies on the dominance relationship in various AD systems. -
dc.identifier.bibliographicCitation JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA, v.25, no.11, pp.2272 - 2280 -
dc.identifier.doi 10.1016/S1001-0742(12)60299-X -
dc.identifier.issn 1001-0742 -
dc.identifier.scopusid 2-s2.0-84887599604 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/4001 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84887599604 -
dc.identifier.wosid 000326994200013 -
dc.language 영어 -
dc.publisher SCIENCE PRESS -
dc.title Absolute dominance of hydrogenotrophic methanogens in full-scale anaerobic sewage sludge digesters -
dc.type Article -
dc.relation.journalWebOfScienceCategory Environmental Sciences -
dc.relation.journalResearchArea Environmental Sciences & Ecology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Anaerobic digestion -
dc.subject.keywordAuthor Hydrogenotrophic methanogens -
dc.subject.keywordAuthor Methanomicrobiales -
dc.subject.keywordAuthor Microbial community structure -
dc.subject.keywordAuthor Sewage sludge -

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