BROWSE

Related Researcher

Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

ITEM VIEW & DOWNLOAD

Precisely targeted delivery of cells and biomolecules within microchannels using aqueous two-phase systems

Cited 10 times inthomson ciCited 9 times inthomson ci
Title
Precisely targeted delivery of cells and biomolecules within microchannels using aqueous two-phase systems
Author
Frampton, John P.Lai, DavidSriram, HariTakayama, Shuichi
Keywords
Microfluidic; Laminar; Droplet; Aqueous two-phase; Dextran; Polyethylene glycol
Issue Date
201112
Publisher
SPRINGER
Citation
BIOMEDICAL MICRODEVICES, v.13, no.6, pp.1043 - 1051
Abstract
Laminar and pulsatile flow of aqueous solutions in microfluidic channels can be useful for controlled delivery of cells and molecules. Dispersion effects resulting from diffusion and convective disturbances, however, result in reagent delivery profiles becoming blurred over the length of the channels. This issue is addressed partially by using oil-in-water phase systems. However, there are limitations in terms of the biocompatibility of these systems for adherent cell culture. Here we present a fully biocompatible aqueous two-phase flow system that can be used to pattern cells within simple microfluidic channel designs, as well as to deliver biochemical treatments to cells according to discrete boundaries. We demonstrate that aqueous two-phase systems are capable of precisely delivering cells as laminar patterns, or as islands by way of forced droplet formation. We also demonstrate that these systems can be used to precisely control chemical delivery to preformed monolayers of cells growing within channels. Treatments containing trypsin were localized more reliably using aqueous two-phase delivery than using conventional delivery in aqueous medium
URI
Go to Link
DOI
http://dx.doi.org/10.1007/s10544-011-9574-y
ISSN
1387-2176
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU