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DC Field | Value | Language |
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dc.citation.endPage | 6907 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 6902 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.contributor.author | Kim, Sung-Jin | - |
dc.contributor.author | Paczesny, Sophie | - |
dc.contributor.author | Takayama, Shuichi | - |
dc.contributor.author | Kurabayashi, Katsuo | - |
dc.date.accessioned | 2023-12-22T03:42:38Z | - |
dc.date.available | 2023-12-22T03:42:38Z | - |
dc.date.created | 2013-08-28 | - |
dc.date.issued | 2013-07 | - |
dc.description.abstract | Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ∼5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.14, pp.6902 - 6907 | - |
dc.identifier.doi | 10.1021/ac401292d | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.scopusid | 2-s2.0-84880569397 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/3771 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84880569397 | - |
dc.identifier.wosid | 000322059600051 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Preprogrammed, parallel on-chip immunoassay using system-level capillarity control | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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