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Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Preprogrammed, parallel on-chip immunoassay using system-level capillarity control

Cited 2 times inthomson ciCited 1 times inthomson ci
Title
Preprogrammed, parallel on-chip immunoassay using system-level capillarity control
Author
Kim, Sung-JinPaczesny, SophieTakayama, ShuichiKurabayashi, Katsuo
Keywords
C-reactive proteins; Capillarity control; Detection channels; Enzyme linked immunosorbent assay; Immunoassay techniques; Micro-fluidic devices; Multiple solutions; On-chip passive components
Issue Date
201307
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.85, no.14, pp.6902 - 6907
Abstract
Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ∼5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources
URI
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DOI
http://dx.doi.org/10.1021/ac401292d
ISSN
0003-2700
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