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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

Cited 58 times inthomson ciCited 45 times inthomson ci
Title
Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering
Author
Yang, KisukLee, Jung SeungKim, JinLee, Yu BinShin, HeungsooUm, Soong HoKim, Jeong BeomPark, Kook InLee, HaeshinCho, Seung-Woo
Keywords
Coating material; Growth factor; Multi-step; Neural stem cell; Neurotrophic; Physical absorption; Platform technology; Pluripotent; Polydopamine; Polydopamine coating; Polymer substrate; Safety issues; Scaffold materials; Stem cell therapy; Surface conjugation; Surface immobilization; Surface modification methods; Therapeutic Application; Tissue engineering scaffold
Issue Date
201210
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS, v.33, no.29, pp.6952 - 6964
Abstract
Surface modification of tissue engineering scaffolds and substrates is required for improving the efficacy of stem cell therapy by generating physicochemical stimulation promoting proliferation and differentiation of stem cells. However, typical surface modification methods including chemical conjugation or physical absorption have several limitations such as multistep, complicated procedures, surface denaturation, batch-to-batch inconsistencies, and low surface conjugation efficiency. In this study, we report a mussel-inspired, biomimetic approach to surface modification for efficient and reliable manipulation of human neural stem cell (NSC) differentiation and proliferation. Our study demonstrates that polydopamine coating facilitates highly efficient, simple immobilization of neurotrophic growth factors and adhesion peptides onto polymer substrates. The growth factor or peptide-immobilized substrates greatly enhance differentiation and proliferation of human NSCs (human fetal brain-derived NSCs and human induced pluripotent stem cell-derived NSCs) at a level comparable or greater than currently available animal-derived coating materials (Matrigel) with safety issues. Therefore, polydopamine-mediated surface modification can provide a versatile platform technology for developing chemically defined, safe, functional substrates and scaffolds for therapeutic applications of human NSCs.
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DOI
http://dx.doi.org/10.1016/j.biomaterials.2012.06.067
ISSN
0142-9612
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