File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.endPage 2134 -
dc.citation.number 9 -
dc.citation.startPage 2129 -
dc.citation.title BIOSENSORS & BIOELECTRONICS -
dc.citation.volume 25 -
dc.contributor.author Dharuman, V. -
dc.contributor.author Chang, B-Y. -
dc.contributor.author Park, Su-Moon -
dc.contributor.author Hahn, J. H. -
dc.date.accessioned 2023-12-22T07:09:36Z -
dc.date.available 2023-12-22T07:09:36Z -
dc.date.created 2013-06-18 -
dc.date.issued 2010-05 -
dc.description.abstract Target recognizing ternary mixed monolayer is constructed by simple sequential adsorption of thiol caped single stranded DNA (HS-ssDNA), 6-mercapto-1-hexanol (MCH) and 3-mercaptopropionic acid (MPA) diluents (abbreviated as HS-ssDNA/MPA/MCH) and applied for detection of DNA hybridization sequence by electrochemical method. The method is more reliable and reproducible on both conventional wire electrode and 100 nm scale gold-coated silicon chips. Detection limit of 10 pM is observed constantly on all types of electrodes. This ternary layer approach provides 60-80% discrimination effect between the hybridized and un-hybridized surfaces compared to the binary mixed monolayers HS-ssDNA/MCH and HS-ssDNA/MPA that showed 20-30% only. Characterization by impedance spectroscopy, X-ray photoelectron spectroscopy and surface Fourier Transform Infrared (FT-IR) techniques reveals 'head-to-head' anisotropic hydrogen bonding between MPA and MCH diluents that controls the HS-ssDNA orientation and enhancing the electrostatic blockade for K(3)[Fe(CN)(6)]. This is the first report characterizing the ternary layer for DNA molecular affinity sensing. Results provided unprecedented insight onto the label free electrochemical sensing and understanding of the complex sensing mechanism to develop sensors that is more reliable. -
dc.identifier.bibliographicCitation BIOSENSORS & BIOELECTRONICS, v.25, no.9, pp.2129 - 2134 -
dc.identifier.doi 10.1016/j.bios.2010.02.019 -
dc.identifier.issn 0956-5663 -
dc.identifier.scopusid 2-s2.0-77951566573 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/3639 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77951566573 -
dc.identifier.wosid 000277930000021 -
dc.language 영어 -
dc.publisher ELSEVIER ADVANCED TECHNOLOGY -
dc.title Ternary mixed monolayers for simultaneous DNA orientation control and surface passivation for label free DNA hybridization electrochemical sensing -
dc.type Article -
dc.relation.journalWebOfScienceCategory Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology -
dc.relation.journalResearchArea Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.