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Author

Kang, Sebyung
Protein Nanobio Lab
Research Interests
  • Protein engineering, Drug/diagnostics delivery nanoplatform, Protein-base vaccine delivery systems, Biosensor & imaging

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Implementation of P22 Viral Capsids as Nanoplatforms

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Title
Implementation of P22 Viral Capsids as Nanoplatforms
Author
Kang, SebyungUchida, MasakiO'Neil, AlisonLi, RuiPrevelige, Peter E.Douglas, Trevor
Keywords
Conformational change; High resolution mass spectrometry; Interior surfaces; Internal modifications; Internal surfaces; Protein complexes; Self-assemble; Site-specific; Streptavidin; Structural flexibilities; Structural transformation; Viral capsids
Issue Date
201010
Publisher
AMER CHEMICAL SOC
Citation
BIOMACROMOLECULES, v.11, no.10, pp.2804 - 2809
Abstract
Viral capsids are dynamic macromolecular machines which self-assemble and undergo concerted conformational changes during their life cycle. We have taken advantage of the inherent structural flexibility of viral capsids and generated two morphologically different types of viral nanoplatforms from the bacteriophage P22 capsids. Their interior surfaces were genetically manipulated For site-specific attachment of 0 biotin linker. The extent of internal modifications in each capsid form was Characterized by high-resolution mass spectrometry and the analyses revealed that the reactivity of the genetically introduced residues located on the internal surface changes according to the structural transformation of the capsid. Internally modified capsids having 10 nm diameter pores at the 12 icosahedral vertices, so-called wiffle-balls (WB), exhibited the capability to entrap the large tetrameric protein complex streptavidin via the biotin linker anchored onto the interior surface of the WB.
URI
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DOI
http://dx.doi.org/10.1021/bm100877q
ISSN
1525-7797
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