Induction of pulmonary mucosal immune responses with a protein vaccine targeted to the DEC-205/CD205 receptor
Cited 0 times inCited 7 times in
- Induction of pulmonary mucosal immune responses with a protein vaccine targeted to the DEC-205/CD205 receptor
- Do, Yoonkyung; Didierlaurent, Arnaud M.; Ryu, Seongho; Koh, Hyein; Park, Chae Gyu; Park, Steven; Perlin, David S.; Powell, Bradford S.; Steinman, Ralph M.
- CD205/DEC-205; Cellular immunity; Dendritic cells; LcrV; Mucosal; Y. pestis
- Issue Date
- ELSEVIER SCI LTD
- VACCINE, v.30, no.45, pp.6359 - 6367
- It is of great interest to develop a pneumonic plague vaccine that would induce combined humoral and cellular immunity in the lung. Here we investigate a novel approach based on targeting of dendritic cells using the DEC-205/CD205 receptor (DEC) via the intranasal route as way to improve mucosal cellular immunity to the vaccine. Intranasal administration of Yersinia pestis LcrV (V) protein fused to anti-DEC antibody together with poly IC as an adjuvant induced high frequencies of IFN-gamma secreting CD4(+) T cells in the airway and lung as well as pulmonary IgG and IgA antibodies. Anti-DEC:LcrV was more efficient to induce IFN-gamma/TNF-alpha/IL-2 secreting polyfunctional CD4(+) T cells when compared to non-targeted soluble protein vaccine. In addition, the intranasal route of immunization with anti-DEC:LcrV was associated with improved survival upon pulmonary challenge with the virulent CO92 Y. pestis. Taken together, these data indicate that targeting dendritic cells via the mucosal route is a potential new avenue for the development of a mucosal vaccine against pneumonic plague.
- ; Go to Link
Appears in Collections:
- SLS_Journal Papers
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.