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Choi, Ung-Kyu
School of Nano-Bioscience and Chemical Engineering
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칼슘함량이 강화된 새송이 버섯의 프로테옴 분석

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Title
칼슘함량이 강화된 새송이 버섯의 프로테옴 분석
Other Titles
Proteomic characteristics of calcium enriched King Oyster Mushroom (Pleurotus eryngii)
Author
Bae, Hee-SunKim, Dae-HyunChoi, Ung-Kyu
Keywords
Species Index: Basidiomycota; Pleurotus eryngii; Pleurotus ostreatus
Issue Date
201102
Publisher
한국식품과학회
Citation
한국식품과학회지, v.43, no.1, pp.12 - 16
Abstract
This study was conducted to identify the differences in proteomic characteristics between Ca-enriched king oyster mushrooms and general king oyster mushrooms. A combined high-throughput proteomic approach was employed to determine the expression profiles and identity of proteins using 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. The overall distribution patterns of the proteins were quite similar, but many of the protein spot intensities varied. A total of 10 proteins, representing a significant difference in the quantities of protein betweenthe two types of mushrooms, were successfully identified. Among these proteins, eight kinds were increased in the Ca-enriched king oyster mushrooms and two kinds were decreased. This study showed that proteomic analysis can help define specific changes in protein level and composition, which can occur in mushrooms where Ca content may or may not be enriched.
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ISSN
0367-6293
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