dc.citation.conferencePlace |
US |
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dc.citation.conferencePlace |
Seattle, WA |
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dc.citation.endPage |
75 |
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dc.citation.startPage |
73 |
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dc.citation.title |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
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dc.contributor.author |
Wang, H |
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dc.contributor.author |
Witek, M |
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dc.contributor.author |
Park, D |
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dc.contributor.author |
Huang, J |
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dc.contributor.author |
Barany, F |
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dc.contributor.author |
Soper, Steven A. |
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dc.date.accessioned |
2023-12-20T02:39:09Z |
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dc.date.available |
2023-12-20T02:39:09Z |
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dc.date.created |
2019-03-26 |
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dc.date.issued |
2011-10-02 |
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dc.description.abstract |
A solid-phase PCR strategy on a novel 3-D substrate consisting of 2 μm SU-8 pillars that are >5 μm in height have been lithographically patterned onto quartz substrates and is reported. The surface chemistry of SU-8 allows the printing of PCR primers only onto the SU-8 pillars but not on the quartz substrate, which creates highly ordered arrays of clusters making imaging of the clonally-amplified DNA using high density CCDs more efficient. The 3D architecture employed herein also provides significantly improved signal-to-noise ratios for the next phase of the assay (e.g. sequencing-by-synthesis (SbS)) in next generation sequencing (NGS) instruments. |
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dc.identifier.bibliographicCitation |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, pp.73 - 75 |
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dc.identifier.scopusid |
2-s2.0-84883750408 |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/35019 |
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dc.language |
영어 |
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dc.publisher |
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
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dc.title |
Novel 3D lithographically-prepared solid-phase surfaces made from SU-8 for next generation sequencing |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2011-10-02 |
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