Microfluidic platform for detecting circulating leukemic cells: Analysis of minimum residual disease in acute myeloid leukemia as a case example

Abstract

We report an integrated microfluidic system for isolating and analyzing circulating leukemic cells (CLCs) from peripheral blood to provide for sensitive minimum residual disease (MRD) diagnostics for acute myeloid leukemia (AML). Microfluidic devices isolate CLCs in microchannels coated with cellspecific antibodies (Abs), which are immobilized onto cleavable linkers. After immunostaining on-chip, linker cleavage releases CLCs to provide for single-cell impedance detection and immunophenotyping in a novel micro multi-parameter flow cytometer (μMFC). All microfluidic devices are integrated to a fluidic motherboard for automated operation that controls fluid distribution. © 14CBMS.