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Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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Sensing of plant hydrolysate-related phenolics with an aaeXAB::luxCDABE bioreporter strain of Escherichia coli

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Title
Sensing of plant hydrolysate-related phenolics with an aaeXAB::luxCDABE bioreporter strain of Escherichia coli
Author
Monnappa, Ajay KalanjanaLee, SiseonMitchell, Robert J.
Keywords
AaeA; E. coli; Ferulic acids; Minimum detectable concentrations; Phenolic; Phenolics; Rice straw hydrolysate; Single compound; Vanillic acids
Issue Date
201301
Publisher
ELSEVIER SCI LTD
Citation
BIORESOURCE TECHNOLOGY, v.127, no., pp.429 - 434
Abstract
A bioluminescent Escherichia coil bioreporter strain to detect hydrolysate related phenolics was developed by cloning the aaeXAB promoter from E. coli upstream of the luxCDABE genes. E. coli str. DH5 alpha carrying this plasmid (pDMA3) was responsive to sub-inhibitory concentrations of plant hydrolysate-related phenolics, such as ferulic and vanillic acids, responding to these compounds at concentrations as low as 9.8 and 4.9 mg/L, respectively. Experiments with a mixture of the compounds showed similar responses as with single compound tests, with a minimum detectable concentration of 19.5 mg/L Finally, tests using rice straw hydrolysates were conducted, with E. coli str. DH5 alpha/pDMA3 showing a maximum induction of 33-fold and a minimum detectable phenolic concentration of 9.3 mg/L, based upon Folin-Ciocalteu's reagent. These results demonstrate that this bioreporter maintains its sensitivity even with hydrolysate samples and that it can be potentially applied within biofuel industries to detect phenolics present within plant hydrolysates.
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DOI
http://dx.doi.org/10.1016/j.biortech.2012.09.086
ISSN
0960-8524
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