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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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On-chip concentration of bacteria using a 3D dielectrophoretic chip and subsequent laser-based DNA extraction in the same chip

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Title
On-chip concentration of bacteria using a 3D dielectrophoretic chip and subsequent laser-based DNA extraction in the same chip
Author
Cho, Yoon-KyoungKim, Tae-hyeongLee, Jeong-Gun
Keywords
3-D electrodes; Au electroplating; Bacteria concentrations; Concentration of; Dielectrophoretic; Dielectrophoretic chip; DNA extraction; Laser irradiations; Magnetic beads; Manual operations; Metal posts; Nonuniform electric field; On chips; Real-time polymerase chain reaction; Silicon chip; Staphylococcus epidermidis; Streptococcus mutans; Three models
Issue Date
201006
Publisher
IOP PUBLISHING LTD
Citation
JOURNAL OF MICROMECHANICS AND MICROENGINEERING, v.20, no.6, pp.1 - 10
Abstract
We report the on-chip concentration of bacteria using a dielectrophoretic (DEP) chip with 3D electrodes and subsequent laser-based DNA extraction in the same chip. The DEP chip has a set of interdigitated Au post electrodes with 50 mu m height to generate a network of non-uniform electric fields for the efficient trapping by DEP. The metal post array was fabricated by photolithography and subsequent Ni and Au electroplating. Three model bacteria samples (Escherichia coli, Staphylococcus epidermidis, Streptococcus mutans) were tested and over 80-fold concentrations were achieved within 2 min. Subsequently, on-chip DNA extraction from the concentrated bacteria in the 3D DEP chip was performed by laser irradiation using the laser-irradiated magnetic bead system (LIMBS) in the same chip. The extracted DNA was analyzed with silicon chip-based real-time polymerase chain reaction (PCR). The total process of on-chip bacteria concentration and the subsequent DNA extraction can be completed within 10 min including the manual operation time.
URI
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DOI
http://dx.doi.org/10.1088/0960-1317/20/6/065010
ISSN
0960-1317
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