BROWSE

Related Researcher

Author

Lee, Chang Young
Carbon Nanomaterials Lab
Research Interests
  • Carbon nanomaterials, gas sensors, neuroanalytical chemistry, biomimetic nanosystems

ITEM VIEW & DOWNLOAD

Microfluidic Device for the Selective Chemical Stimulation of Neurons and Characterization of Peptide Release with Mass Spectrometry

Cited 0 times inthomson ciCited 5 times inthomson ci
Title
Microfluidic Device for the Selective Chemical Stimulation of Neurons and Characterization of Peptide Release with Mass Spectrometry
Author
Croushore, Callie A.Supharoek, Sam-angLee, Chang YoungJakmunee, JaroonSweedler, Jonathan V.
Keywords
Aplysia californica; Chemical solutions; Chemical stimulation; Extracellular environments; Low density; Mass-limited; Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; Micro fluidic system; Micro-fluidic devices; Neuronal networks; Neuropeptides; Peptide release; Physiological process; Small samples; Temporal pattern
Issue Date
201211
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.84, no.21, pp.9446 - 9452
Abstract
Neuropeptides are synthesized in and released from neurons and are involved in a wide range of physiological processes, including temperature homeostasis, learning, memory, and disease. When working with sparse neuronal. net-works, the ability to collect and characterize small sample volumes is important as neurons often release only a small proportion of their mass limited content Microfluidic systems are well suited for the study of neuropeptides. They offer the ability to control and manipulate the extracellular environment and small sample volumes, thereby reducing the dilution of peptides following release. We present an approach for the culture and stimulation of a neuronal network within a microfluidic device, subsequent collection of the released peptides, and their detection via mass spectrometry. The system employs microvalve-controlled stimulation channels to selectively stimulate a low-density neuronal culture, allowing us to determine the temporal onset of peptide release. Released peptides from the well-characterized, peptidergic bag cell neurons of Aplysia californica were collected and their temporal pattern of release was characterized with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We show a robust difference in the timing of release for chemical solutions containing elevated K+ (7 +/- 3 min), when compared to insulin (19 +/- 7 min) (p < 0.00001).
URI
Go to Link
DOI
http://dx.doi.org/10.1021/ac302283u
ISSN
0003-2700
Appears in Collections:
ECHE_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU