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Lee, Sung Kuk
Synthetic Biology Laboratory
Research Interests
  • - Design and optimization of metabolic pathways for value-added products

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Transcriptome-driven synthetic re-modeling of Escherichia coli to enhance cellobiose utilization

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Title
Transcriptome-driven synthetic re-modeling of Escherichia coli to enhance cellobiose utilization
Author
Parisutham, VinuselviJung, Sang-KyuNam, DouguLee, Sung Kuk
Keywords
Asc operon; Cellobiose metabolism; Chb operon; Escherichia coli; Lignocellulosic biomass; Transcriptome
Issue Date
201311
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
CHEMICAL ENGINEERING SCIENCE, v.103, no., pp.50 - 57
Abstract
An efficient cellobiose-metabolizing strain of Escherichia coli would provide several industrial benefits, particularly with lignocellulosic biomass substrates. An efficient cellobiose-metabolizing E. coli strain (without the introduction of heterologous genes) was constructed by a combination of promoter engineering of cryptic operons (chb and asc) and adaptive evolution. Gene expression patterns in rapid and slow cellobiose-metabolizing strains were analyzed using DNA microarray. Genes involved in nucleotide biosynthesis, ATP generation, and flagellar assembly were upregulated following adaptation, indicating the possibility of active metabolism and fast growth. On the other hand, genes involved in chemotaxis toward glucose and response to starvation and other stresses were downregulated. No significant changes were observed in the expression of genes regulated by cyclic AMP receptor protein (CRP), the global regulator of carbon metabolism. Overexpression of ascB, which is located in the minor cryptic operon, seems to play a vital role in enhancing cellobiose metabolism. Changes in ascB transcription were confirmed by semiquantitative RT-PCR. The importance of ascB for cell growth on cellobiose was also validated by overexpression and knockout studies. Our study provides new insights into the role of the chb and asc operons in cellobiose metabolism.
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DOI
http://dx.doi.org/10.1016/j.ces.2012.08.006
ISSN
0009-2509
Appears in Collections:
ECHE_Journal Papers
SLS_Journal Papers

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