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Author

Lee, Sung Kuk
Synthetic Biology Laboratory
Research Interests
  • - Design and optimization of metabolic pathways for value-added products

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Engineered Escherichia coli capable of co-utilization of cellobiose and xylose

Cited 7 times inthomson ciCited 5 times inthomson ci
Title
Engineered Escherichia coli capable of co-utilization of cellobiose and xylose
Author
Parisutham, VinuselviLee, Sung Kuk
Keywords
Biofuel production; Cellobiose; Co-metabolism; Co-utilization; Cofermentation; Consolidated bioprocessing; E. coli; Evolutionary engineering; Plant biomass; Recombinant E. coli; Simultaneous saccharification and co-fermentation; Wild types
Issue Date
201201
Publisher
ELSEVIER SCIENCE INC
Citation
ENZYME AND MICROBIAL TECHNOLOGY, v.50, no.1, pp.1 - 4
Abstract
Natural ability to ferment the major sugars (glucose and xylose) of plant biomass is an advantageous feature of Escherichia coil in biofuel production. However, excess glucose completely inhibits xylose utilization in E. coil and decreases yield and productivity of fermentation due to sequential utilization of xylose after glucose. As an approach to overcome this drawback, E. coil MG1655 was engineered for simultaneous glucose (in the form of cellobiose) and xylose utilization by a combination of genetic and evolutionary engineering strategies. The recombinant E. coli was capable of utilizing approximately 6 g/L of cellobiose and 2 g/L of xylose in approximately 36 h, whereas wild-type E. coil was unable to utilize xylose completely in the presence of 6 g/L of glucose even after 75 hours. The engineered strain also co-utilized cellobiose with mannose or galactose; however, it was unable to metabolize cellobiose in the presence of arabinose and glucose. Successful cellobiose and xylose co-fermentation is a vital step for simultaneous saccharification and co-fermentation process and a promising step towards consolidated bioprocessing.
URI
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DOI
http://dx.doi.org/10.1016/j.enzmictec.2011.10.001
ISSN
0141-0229
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