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Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds

Cited 4 times inthomson ciCited 3 times inthomson ci
Title
Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds
Author
Lee, SiseonNam, DouguJung, Joon YoungOh, Min-KyuSang, Byoung-InMitchell, Robert J.
Keywords
4-hydroxybenzoic acids; Aromatic carboxylic acids; Coumaric acid; Dose-dependent; E. coli; Expression levels; Ferulic acids; Heat shock response; Hydrolysate; Transcriptomes; Vanillic acids
Issue Date
201206
Publisher
ELSEVIER SCI LTD
Citation
BIORESOURCE TECHNOLOGY, v.114, no., pp.450 - 456
Abstract
Aberrations in the growth and transcriptome of Escherichia coli str. BL21(DE3) were determined when exposed to varying concentrations of ferulic acid (0.25-1 g/L), an aromatic carboxylic acid identified within lignin-cellulose hydrolysate samples. The expression of several individual genes (aaeA, aaeB, inaA and marA) was significantly induced, i.e., more than 4-fold, and thus these genes and the heat shock response gene htpG were selected as biomarkers to monitor E. coli's responses to five additional hydrolysate-related compounds, including vanillic acid, coumaric acid, 4-hydroxybenzoic acid, ferulaldehyde and furfural. While all of the biomarkers showed dose-dependent responses to most of the compounds, expression of aaeA and aaeB showed the greatest induction (5-30-fold) for all compounds tested except furfural. Lastly, the marA, inaA and htpG genes all showed higher expression levels when the culture was exposed to spruce hydrolysate samples, demonstrating the potential use of these genes as biomarkers.
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DOI
http://dx.doi.org/10.1016/j.biortech.2012.02.085
ISSN
0960-8524
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