MRI size assessment of cerebral microvasculature using diffusion-time-dependent stimulated-echo acquisition: A feasibility study in rodent

Abstract

In this study, a stimulated-echo (STE) method was employed to robustify the cerebral vessel size estimation nearair-tissue, bone-tissue interfaces, and large vessels. The proposed solution is to replace the relaxation rate changefrom gradient-echo (GRE) with that from STE with long diffusion time after the injection of an intravascularcontrast agent, superparamagnetic iron oxide nanoparticles. The corresponding diffusion length of STE is shorterthan the length over which the unwanted macroscopicfield inhomogeneities but is still longer than the corre-lation length of thefields induced by small vessels. Therefore, the unwantedfield inhomogeneities are refocused,while preserving microscopic susceptibility contrast from cerebral vessels. The mean vessel diameter (dimen-sionless) derived from the diffusion-time-varying STE method was compared to the mean vessel diameter ob-tained by a conventional spin-echo (SE) and GRE combination based on Monte-Carlo proton diffusion simulationsand in vivo rat experiments at 7 T. The in vivo mean vessel diameter from the MRI experiments was directlycompared to available reference mouse brain vasculature obtained by a knife-edge scanning microscope (KESM),which is considered to be the gold standard. Monte-Carlo simulation revealed that SE and GRE-based MRrelaxation rate changes (ΔR2andΔR2*, respectively) can be enhanced using single STE-based MR relaxation ratechange (ΔRSTE) by regulating diffusion time, especially for small vessels. The in vivo mean vessel diameter fromthe STE method demonstrated a closer agreement with that from the KESM compared to the combined SE andGRE method, especially in the olfactory bulb and cortex. This study demonstrates that STE relaxation rate changescan be used as consistent measures for assessing small cerebral microvasculature, where macroscopicfield in-homogeneity is severe and signal contamination from adjacent large vessels is significant.