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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 278 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 269 | - |
dc.citation.title | OPTICS COMMUNICATIONS | - |
dc.citation.volume | 272 | - |
dc.contributor.author | Leray, A. | - |
dc.contributor.author | Odin, C. | - |
dc.contributor.author | Huguet, E. | - |
dc.contributor.author | Amblard, F. | - |
dc.contributor.author | Le Grand, Y. | - |
dc.date.accessioned | 2023-12-22T09:16:06Z | - |
dc.date.available | 2023-12-22T09:16:06Z | - |
dc.date.created | 2020-01-31 | - |
dc.date.issued | 2007-04 | - |
dc.description.abstract | We report on measurements and Monte Carlo analysis of the spatial distribution of light in two-photon excitation fluorescence (TPEF) microscopy operating through scattering media. The axial profile of TPEF produced by a high numerical aperture microscope objective focusing through a tissue-like optical phantom at depths more than five photon mean-scattering lengths is studied. The measured profile is quantitatively interpreted with time-resolved Monte Carlo simulations that take into account the spatio-temporal distribution of the photons within the turbid medium under a femtosecond excitation regime. It is shown that considering only the ballistic photons leads to an under-estimation of the actual out-of-focus distribution of the fluorescence, whereas it is over-estimated when the ballistic and scattered photons are treated alike. Comparison of the in-focus signal to the overall out-of-focus background clearly pointed out that the signal-to-background ratio of TPEF microscopy images benefits from a shortening of the excitation pulses. Moreover, in homogeneously labeled specimen, the background is shown to overcome the signal at imaging depths of about five to six photon mean-scattering paths. | - |
dc.identifier.bibliographicCitation | OPTICS COMMUNICATIONS, v.272, no.1, pp.269 - 278 | - |
dc.identifier.doi | 10.1016/j.optcom.2006.11.007 | - |
dc.identifier.issn | 0030-4018 | - |
dc.identifier.scopusid | 2-s2.0-33846185542 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/31064 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0030401806011941?via%3Dihub | - |
dc.identifier.wosid | 000244727200044 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Spatially distributed two-photon excitation fluorescence in scattering media: Experiments and time-resolved Monte Carlo simulations | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.relation.journalResearchArea | Optics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | OPTICAL-PROPERTIES | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | LASER | - |
dc.subject.keywordPlus | PROPAGATION | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | TISSUES | - |
dc.subject.keywordPlus | PHOTON | - |
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