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DC Field | Value | Language |
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dc.citation.number | 7 | - |
dc.citation.startPage | e1001025. | - |
dc.citation.title | PLOS GENETICS | - |
dc.citation.volume | 6 | - |
dc.contributor.author | Bailly, Aymeric P. | - |
dc.contributor.author | Freeman, Alasdair | - |
dc.contributor.author | Hall, Julie | - |
dc.contributor.author | Declais, Anne-Cecile | - |
dc.contributor.author | Alpi, Arno | - |
dc.contributor.author | Lilley, David M. J. | - |
dc.contributor.author | Ahmed, Shawn | - |
dc.contributor.author | Gartner, Anton | - |
dc.date.accessioned | 2023-12-22T07:06:46Z | - |
dc.date.available | 2023-12-22T07:06:46Z | - |
dc.date.created | 2020-01-30 | - |
dc.date.issued | 2010-07 | - |
dc.description.abstract | DNA double-strand breaks (DSBs) can be repaired by homologous recombination (HR), which can involve Holliday junction (HJ) intermediates that are ultimately resolved by nucleolytic enzymes. An N-terminal fragment of human GEN1 has recently been shown to act as a Holliday junction resolvase, but little is known about the role of GEN-1 in vivo. Holliday junction resolution signifies the completion of DNA repair, a step that may be coupled to signaling proteins that regulate cell cycle progression in response to DNA damage. Using forward genetic approaches, we identified a Caenorhabditis elegans dual function DNA double-strand break repair and DNA damage signaling protein orthologous to the human GEN1 Holliday junction resolving enzyme. GEN-1 has biochemical activities related to the human enzyme and facilitates repair of DNA double-strand breaks, but is not essential for DNA double-strand break repair during meiotic recombination. Mutational analysis reveals that the DNA damage-signaling function of GEN-1 is separable from its role in DNA repair. GEN-1 promotes germ cell cycle arrest and apoptosis via a pathway that acts in parallel to the canonical DNA damage response pathway mediated by RPA loading, CHK1 activation, and CEP-1/p53-mediated apoptosis induction. Furthermore, GEN-1 acts redundantly with the 9-1-1 complex to ensure genome stability. Our study suggests that GEN-1 might act as a dual function Holliday junction resolvase that may coordinate DNA damage signaling with a late step in DNA double-strand break repair. | - |
dc.identifier.bibliographicCitation | PLOS GENETICS, v.6, no.7, pp.e1001025. | - |
dc.identifier.doi | 10.1371/journal.pgen.1001025 | - |
dc.identifier.issn | 1553-7404 | - |
dc.identifier.scopusid | 2-s2.0-77957375933 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30999 | - |
dc.identifier.url | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1001025 | - |
dc.identifier.wosid | 000280512700021 | - |
dc.language | 영어 | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | The Caenorhabditis elegans Homolog of Gen1/Yen1 Resolvases Links DNA Damage Signaling to DNA Double-Strand Break Repair | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | CHECKPOINT PROTEIN | - |
dc.subject.keywordPlus | GERM-LINE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | TELOMERE REPLICATION | - |
dc.subject.keywordPlus | HOLLIDAY JUNCTION RESOLVASE | - |
dc.subject.keywordPlus | STRUCTURE-SPECIFIC NUCLEASES | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCISION-REPAIR | - |
dc.subject.keywordPlus | CELL-CYCLE ARREST | - |
dc.subject.keywordPlus | C-ELEGANS | - |
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