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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Bacteria concentration using a membrane type insulator-based dielectrophoresis in a plastic chip

Cited 34 times inthomson ciCited 34 times inthomson ci
Title
Bacteria concentration using a membrane type insulator-based dielectrophoresis in a plastic chip
Author
Cho, Yoon-KyoungKim, SuhyeonLee, KyusangPark, ChinsungLee, Jeong-GunKo, Christopher
Keywords
Bacteria concentration; Dielectrophoresis; Insulator-based dielectrophoresis; Microfabrication
Issue Date
200909
Publisher
WILEY-V C H VERLAG GMBH
Citation
ELECTROPHORESIS, v.30, no.18, pp.3153 - 3159
Abstract
We report an insulator-based (or, electrodeless) dielectrophoresis utilizing microfabricated plastic membranes. The membranes with honeycomb-type pores have been fabricated by patterning the SU-8 layer on a substrate which was pretreated with self-assembled monolayer of octadecyltrichlorosilane for the easy release. The fabricated membrane was positioned between two electrodes and alternating current field was applied for the particle trap experiments. The particle could be trapped due to the dielectrophoresis force generated by the non-uniformities of the electric fields applied through the membranes with pores. Simulations using CFD-ACE+ (CFD Research, Huntsville, Alabama) suggested that the dielectrophoresis force is stronger in the edge of the pores where the field gradient is highest. The bacteria could be captured on the near edge of the pores when the electric field was turned on and the trapped bacteria could be released when the field was turned off with the release efficiency of more than 93 +/- 7%. The maximal trapping efficiency of 66 +/- 7% was obtained under the electric fields (E = 128 V/mm and f = 300 kHz) when the dilute bacteria solution (Escherichia coli: 9.3 x 10(3) cell/mL, 0.5 mS/m) flowed with a flow rate of 100 mu L/min.
URI
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DOI
http://dx.doi.org/10.1002/elps.200900179
ISSN
0173-0835
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