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DC Field | Value | Language |
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dc.citation.endPage | 94 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 90 | - |
dc.citation.title | JOURNAL OF IMMUNOLOGICAL METHODS | - |
dc.citation.volume | 363 | - |
dc.contributor.author | Kim, Wonyoung | - |
dc.contributor.author | Kim, Juyang | - |
dc.contributor.author | Shin, Changshik | - |
dc.contributor.author | Kim, Hyunju | - |
dc.contributor.author | Do, Yoonkyung | - |
dc.contributor.author | Cho, Hong R. | - |
dc.contributor.author | Kwon, Byungsuk | - |
dc.date.accessioned | 2023-12-22T06:39:34Z | - |
dc.date.available | 2023-12-22T06:39:34Z | - |
dc.date.created | 2013-06-07 | - |
dc.date.issued | 2010-12 | - |
dc.description.abstract | Eosinophils are rare hematopietic cells that normally constitute only 1 similar to 3% of peripheral blood leukocytes. It would be of help for the purpose of research to obtain a large quantity of eosinophils. In this study, we wanted to develop a novel strategy to induce massive expansion of murine eosinophils in vivo, based on the observation showing that treatment of IL-33 induces eosinophilia in mice. We generated an EL-4 lymphoma cell line (herein named EL-4-IL-33) that was engineered to secrete an active form of IL-33. We found that Siglec-F(+) granulocyte numbers increased by 1850-fold in the peritoneal cavity 10 days after inoculation with 1 x 107 EL-4-IL-33 cells. This number corresponds to 74-fold increase, as compared with the number of Siglec-F(+) granulocytes in mice that received wild-type EL-4 cells. Siglec-F(+) granulocytes expanded by IL-33 had the circular nucleus and expressed eosinophil-specific genes. They also showed some functional characteristics of eosinophils in that they had the ability to respond to IL-5 for survival and eotaxin-1 for chemoattaxis and to produce bioactive eosinophil peroxidase, suggesting that these cells are genuine eosinophils. Our results indicate that sustained secretion of IL-33 by lymphoma cells in the peritoneal cavity is highly effective in increasing peritoneal eosinophil numbers. Therefore, our simple method to obtain eosinphils on a large scale might be of value for eosinophil studies. | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGICAL METHODS, v.363, no.1, pp.90 - 94 | - |
dc.identifier.doi | 10.1016/j.jim.2010.09.033 | - |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.scopusid | 2-s2.0-78649717034 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/3034 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78649717034 | - |
dc.identifier.wosid | 000285950800010 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | A novel method for procuring a large quantity of mature murine eosinophils in vivo | - |
dc.type | Article | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Immunology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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