There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 363 | - |
dc.citation.startPage | 353 | - |
dc.citation.title | EBIOMEDICINE | - |
dc.citation.volume | 48 | - |
dc.contributor.author | Seo, Jae Ho | - |
dc.contributor.author | Agarwal, Ekta | - |
dc.contributor.author | Chae, Young Chan | - |
dc.contributor.author | Lee, Yu Geon | - |
dc.contributor.author | Garlick, David S. | - |
dc.contributor.author | Storaci, Alessandra Maria | - |
dc.contributor.author | Ferrero, Stefano | - |
dc.contributor.author | Gaudioso, Gabriella | - |
dc.contributor.author | Gianelli, Umberto | - |
dc.contributor.author | Vaira, Valentina | - |
dc.contributor.author | Altieri, Dario C. | - |
dc.date.accessioned | 2023-12-21T18:38:21Z | - |
dc.date.available | 2023-12-21T18:38:21Z | - |
dc.date.created | 2019-11-12 | - |
dc.date.issued | 2019-10 | - |
dc.description.abstract | Background: Mitochondrial functions are exploited in cancer and provide a validated therapeutic target. However, how this process is regulated has remained mostly elusive and the identification of new pathways that control mitochondrial integrity in cancer is an urgent priority. Methods: We studied clinically-annotated patient series of primary and metastatic prostate cancer, representative cases of multiple myeloma (MM) and publicly available genetic databases. Gene regulation studies involved chromatin immunoprecipitation, PCR amplification and Western blotting of conditional Myc-expressing cell lines. Transient or stable gene silencing was used to quantify mitochondrial functions in bioenergetics, outer membrane permeability, Ca2+ homeostasis, redox balance and cell death. Tumorigenicity was assessed by cell proliferation, colony formation and xenograft tumour growth. Findings: We identified Mitochondrial Fission Factor (MFF) as a novel transcriptional target of oncogenic Myc overexpressed in primary and metastatic cancer, compared to normal tissues. Biochemically, MFF isoforms, MFF1 and MFF2 associate with the Voltage-Dependent Anion Channel-1 (VDAC1) at the mitochondrial outer membrane, in vivo. Disruption of this complex by MFF silencing induces general collapse of mitochondrial functions with increased outer membrane permeability, loss of inner membrane potential, Ca2+ unbalance, bioenergetics defects and activation of cell death pathways. In turn, this inhibits tumour cell proliferation, suppresses colony formation and reduces xenograft tumour growth in mice. Interpretation: An MFF-VDAC1 complex is a novel regulator of mitochondrial integrity and actionable therapeutic target in cancer. |
- |
dc.identifier.bibliographicCitation | EBIOMEDICINE, v.48, pp.353 - 363 | - |
dc.identifier.doi | 10.1016/j.ebiom.2019.09.017 | - |
dc.identifier.issn | 2352-3964 | - |
dc.identifier.scopusid | 2-s2.0-85074011117 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/30343 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S2352396419306152?via%3Dihub | - |
dc.identifier.wosid | 000493830800039 | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Mitochondrial fission factor is a novel Myc-dependent regulator of mitochondrial permeability in cancer | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal; Medicine, Research & Experimental | - |
dc.relation.journalResearchArea | General & Internal Medicine; Research & Experimental Medicine | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Mitochondria | - |
dc.subject.keywordAuthor | MFF | - |
dc.subject.keywordAuthor | Cell death | - |
dc.subject.keywordAuthor | Tumour metabolism | - |
dc.subject.keywordAuthor | VDAC1 | - |
dc.subject.keywordAuthor | Cancer therapy | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | TRANSITION | - |
dc.subject.keywordPlus | BCL-2 | - |
dc.subject.keywordPlus | DRP1 | - |
dc.subject.keywordPlus | MFF | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | MACHINERY | - |
dc.subject.keywordPlus | NECROSIS | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.