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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Subtype-specific role of phospholipase C-beta in bradykinin and LPA signaling through differential binding of different PDZ scaffold proteins

Cited 19 times inthomson ciCited 20 times inthomson ci
Title
Subtype-specific role of phospholipase C-beta in bradykinin and LPA signaling through differential binding of different PDZ scaffold proteins
Author
Choi, Jung WoongLim, SeyoungOh, Yong-SeokKim, Eung-KyunKim, Sun-HeeKim, Yun-HeeHeo, KyunKim, JaeyoonKim, Jung KukYang, Yong RyulRyu, Sung HoSuh, Pann-Ghill
Keywords
Bradykinin; G protein-coupled receptor; Lysophosphatidic acid; PDZ scaffold; Phospholipase C-β
Issue Date
201007
Publisher
ELSEVIER SCIENCE INC
Citation
CELLULAR SIGNALLING, v.22, no.7, pp.1153 - 1161
Abstract
Among phospholipase C (PLC) isozymes (beta, gamma, delta, epsilon, zeta and eta), PLC-beta plays a key role in G-protein coupled receptor (GPCR)-mediated signaling. PLC-beta subtypes are often overlapped in their distribution, but have unique knock-out phenotypes in organism, suggesting that each subtype may have the different role even within the same type of cells. In this study, we examined the possibility of the differential coupling of each PLC-beta subtype to GPCRs, and explored the molecular mechanism underlying the specificity. Firstly, we found that PLC-beta 1 and PLC-beta 3 are activated by bradykinin (BK) or lysophosphatidic acid (LPA), respectively. BK-triggered phosphoinositides hydrolysis and subsequent Ca(2+) mobilization were abolished specifically by PLC-beta 1 silencing, whereas LPA-triggered events were by PLC-beta 3 silencing. Secondly, we showed the evidence that PDZ scaffold proteins is a key mediator for the selective coupling between PLC-beta subtype and GPCR. We found PAR-3 mediates physical interaction between PLC-beta 1 and BK receptor, while NHERF2 does between PLC-beta 3 and LPA(2) receptor. Consistently, the silencing of PAR-3 or NHERF2 blunted PLC signaling induced by BK or LPA respectively. Taken together, these data suggest that each subtype of PLC-beta is selectively coupled to GPCR via PDZ scaffold proteins in given cell types and plays differential role in the signaling of various GPCRs.
URI
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DOI
http://dx.doi.org/10.1016/j.cellsig.2010.03.010
ISSN
0898-6568
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