BROWSE

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Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures

Cited 7 times inthomson ciCited 6 times inthomson ci
Title
Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures
Author
Torisawa, Yu-SukeMosadegh, BobakCavnar, Stephen P.Ho, MitchellTakayama, Shuichi
Keywords
Cell differentiation; Cell shapes; Cell-cell contact; Cell-cell interaction; Cell-patterning; Cellular pattern; Co-cultures; Coculture; Embryonic stems; Filter membrane; Geometric feature; HepG2 cells; Hydrodynamic forces; mES cells; Micropatterned; Micropatterns; Pdms stamps; Polydimethylsiloxane PDMS
Issue Date
201101
Publisher
MARY ANN LIEBERT INC
Citation
TISSUE ENGINEERING PART C-METHODS, v.17, no.1, pp.61 - 67
Abstract
This article describes a simple and rapid cell patterning method to form co-culture microarrays in commercially available Transwells. A thin poly(dimethylsiloxane) (PDMS) layer is printed on the underside of a Transwell using a PDMS stamp. Arbitrary cellular patterns are generated according to the geometric features of the thin PDMS layer through hydrodynamic forces that guide cells onto the membrane only over the PDMS-uncoated regions. Micropatterns of surface-adhered cells (we refer to this as two-dimensional) or non-surface-adhered clusters of cells (we refer to this as three-dimensional) can be generated depending on the surface treatment of the filter membrane. Additionally, co-cultures can be established by introducing different types of cells on the membrane or in the bottom chamber of the Transwell. We show that this co-culture method can evaluate mouse embryonic stem (mES) cell differentiation based on heterogeneous cell-cell interactions. Co-culture of mES cells and HepG2 cells decreased SOX17 expression of mES cells, and direct cell-cell contact further decreased SOX17 expression, indicating that co-culture with HepG2 cells inhibits endoderm differentiation through soluble factors and cell-cell contact. This method is simple and user-friendly and should be broadly useful to study cell shapes and cell-cell interactions
URI
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DOI
http://dx.doi.org/10.1089/ten.tec.2010.0305
ISSN
1937-3384
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