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Author

Jung, Yun Kyung
Soft and Hybrid Nanomaterials Lab
Research Interests
  • Polymer-based Biosensor

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Highly Tunable Aptasensing Microarrays with Graphene Oxide Multilayers

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Title
Highly Tunable Aptasensing Microarrays with Graphene Oxide Multilayers
Author
Jung, Yun KyungLee, TaeminShin, EeseulKim, Byeong-Su
Keywords
RESONANCE ENERGY-TRANSFER; SENSING BIOMOLECULES; DNA MICROARRAY; LABEL-FREE; FLUORESCENCE; PLATFORM; BIOSENSOR; LIGANDS; COMPLEX; HYBRIDIZATION
Issue Date
201311
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.3, no., pp.3367 -
Abstract
A highly tunable layer-by-layer (LbL)-assembled graphene oxide (GO) array has been devised for high-throughput multiplex protein sensing. In this array, the fluorescence of different target-bound aptamers labeled with dye is efficiently quenched by GO through fluorescence resonance energy transfer (FRET), and simultaneous multiplex target detection is performed by recovering the quenched fluorescence caused by specific binding between an aptamer and a protein. Thin GO films consisting of 10 bilayers displayed a high quenching ability, yielding over 85% fluorescence quenching with the addition of a 2 mu M dye-labeled aptamer. The limit for human thrombin detection in the 6- and 10-bilayered GO array is estimated to be 0.1 and 0.001 nM, respectively, indicating highly tunable nature of LbL assembled GO multilayers in controlling the sensitivity of graphene-based FRET aptasensor. Furthermore, the GO chip could be reused up to four times simply by cleaning it with distilled water.
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DOI
http://dx.doi.org/10.1038/srep03367
ISSN
2045-2322
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