BROWSE

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Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells

Cited 6 times inthomson ciCited 2 times inthomson ci
Title
Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells
Author
Monnappa, Ajay K.Dwidar, MohammedSeo, Jeong KonHur, Jin-HoeMitchell, Robert J.
Keywords
EPIDERMIDIS BIOFILMS; CHEMICAL-COMPOSITION; ENZYMATIC TREATMENTS; BACTERIAL PREDATION; IMMUNE-RESPONSE; PROTEINS; ENZYMES; POLYSACCHARIDE; SUSCEPTIBILITY; FIBRONECTIN
Issue Date
201401
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.4, no., pp.1 - 8
Abstract
Bdellovibrio bacteriovorus HD100 is a predatory bacterium that attacks many Gram-negative human pathogens. A serious drawback of this strain, however, is its ineffectiveness against Gram-positive strains, such as the human pathogen Staphylococcus aureus. Here we demonstrate that the extracellular proteases produced by a host-independent B. bacteriovorus (HIB) effectively degrade/inhibit the formation of S. aureus biofilms and reduce its virulence. A 10% addition of HIB supernatant caused a 75% or greater reduction in S. aureus biofilm formation as well as 75% dispersal of pre-formed biofilms. LC-MS-MS analyses identified various B. bacteriovorus proteases within the supernatant, including the serine proteases Bd2269 and Bd2321. Tests with AEBSF confirmed that serine proteases were active in the supernatant and that they impacted S. aureus biofilm formation. The supernatant also possessed a slight DNAse activity. Furthermore, treatment of planktonic S. aureus with the supernatant diminished its ability to invade MCF-10a epithelial cells by 5-fold but did not affect the MCF-10a viability. In conclusion, this study illustrates the hitherto unknown ability of B. bacteriovorus to disperse Gram-positive pathogenic biofilms and mitigate their virulence.
URI
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DOI
http://dx.doi.org/10.1038/srep03811
ISSN
2045-2322
Appears in Collections:
UCRF_Journal Papers
SLS_Journal Papers
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