BROWSE

Related Researcher

Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

ITEM VIEW & DOWNLOAD

A fully automated immunoassay from whole blood on a disc

Cited 110 times inthomson ciCited 114 times inthomson ci
Title
A fully automated immunoassay from whole blood on a disc
Author
Lee, Beom SeokLee, Jung-NamPark, Jong-MyeonLee, Jeong-GunKim, SuhyeonCho, Yoon-KyoungKo, Christopher
Keywords
LINKED-IMMUNOSORBENT-ASSAY; MICROFLUIDIC DEVICE; MICROCHIP; SYSTEM; EXTRACTION; PLATFORM; PROTEIN; CHIP; BEADS
Issue Date
200906
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.9, no.11, pp.1548 - 1555
Abstract
A portable, disc-based, and fully automated enzyme-linked immuno-sorbent assay (ELISA) system is developed to test infectious diseases from whole blood. The innovative laser irradiated ferrowax microvalves and centrifugal microfluidics were utilized for the full integration of microbead-based suspension ELISA assays on a disc starting from whole blood. The concentrations of the antigen and the antibody of Hepatitis B virus (HBV), HBsAg and Anti-HBs respectively, were measured using the lab-on-a-disc (LOD). All the necessary reagents are preloaded on the disc and the total process of the plasma separation, incubation with target specific antigen or antibody coated microbeads, multiple steps of washing, enzyme reaction with substrates, and the absorbance detection could be finished within 30 minutes. Compared to the conventional ELISA, the operation time was dramatically reduced from over 2 hours to less than 30 minutes while the limit of detection was kept similar; e. g. the limit of detection of Anti-HBs tests were 8.6 mIU mL(-1) and 10 mIU mL(-1) for the disc-based and the conventional ELISA respectively.
URI
Go to Link
DOI
http://dx.doi.org/10.1039/b820321k
ISSN
1473-0197
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU