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Author

Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves

Cited 18 times inthomson ciCited 16 times inthomson ci
Title
Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves
Author
Mosadegh, BobakAgarwal, MayankTavana, HosseinBersano-Begey, TommasoTorisawa, Yu-sukeMorell, MariaWyatt, Matthew J.O'Shea, K. SueBarald, Kate F.Takayama, Shuichi
Keywords
EMTREE medical terms: animal cell; article; device; diffusion; filter; microfluidic analysis; microfluidics; mouse; nonhuman; priority journal; shear stress; valve
Issue Date
2010
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.10, no.21, pp.2959 - 2964
Abstract
Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells
URI
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DOI
http://dx.doi.org/10.1039/c0lc00086h
ISSN
1473-0197
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