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dc.citation.endPage 164 -
dc.citation.startPage 151 -
dc.citation.title FISH & SHELLFISH IMMUNOLOGY -
dc.citation.volume 92 -
dc.contributor.author Kim, Daniel -
dc.contributor.author Lee, Soon Ho -
dc.contributor.author Lee, Hayoung -
dc.contributor.author Kim, Seong-jung -
dc.contributor.author Lee, Kwan Hee -
dc.contributor.author Song, Seong Kyu -
dc.date.accessioned 2023-12-21T18:44:25Z -
dc.date.available 2023-12-21T18:44:25Z -
dc.date.created 2019-09-19 -
dc.date.issued 2019-09 -
dc.description.abstract IL-12 is an important cytokine that connects the innate and adaptive immune systems. The complete gene structure of olive flounder IL-12 and its characteristics have not yet been formally reported. Here, we report the complete sequences of both subunits of olive flounder IL-12 (IL-12p35 and IL-12p40). In addition, its function was analyzed by generating the single-chain rIL-12 of which subunits were fused by a GS linker and the rIL-12-specific mouse antibody. The cDNA sequences of IL-12p35 and IL-12p40 were 1059 nucleotides and 1319 nucleotides, respectively. The analyses of their gene structures, deduced amino acid sequences, protein model structures, and phylogenetic trees confirmed the accurate identification of olive flounder IL-12. The protein structure model suggested that an inter-subunit disulfide bond might be formed between the Cys177 of p35 and Cys74 of p40 to link the subunits. Olive flounder expressed IL-12p40 at higher levels than IL-12p35 in the various tissues under natural conditions although both expression levels were low. However, when infected by Edwardsiella tarda or stimulated by LPS, the flounder expressed both of the subunit genes at similar maximized levels in 6 h and gradually reduced thereafter. Olive flounder PBMC induced with the rIL-12 increased IFN-gamma and TNF-alpha expression but decreased IL-10 expression as did treatment with LPS. However, when the LPS-treated PBMC were neutralized with the rIL-12-specific antibody, the pattern of cytokine expression was precisely reversed. In conclusion, we have formally identified the gene structure and function of olive flounder IL-12. -
dc.identifier.bibliographicCitation FISH & SHELLFISH IMMUNOLOGY, v.92, pp.151 - 164 -
dc.identifier.doi 10.1016/j.fsi.2019.05.033 -
dc.identifier.issn 1050-4648 -
dc.identifier.scopusid 2-s2.0-85066824025 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/27525 -
dc.identifier.url https://www.sciencedirect.com/science/article/pii/S1050464819305893?via%3Dihub -
dc.identifier.wosid 000483634300019 -
dc.language 영어 -
dc.publisher ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD -
dc.title Analyses of the gene structure and function of olive flounder (Paralichthys olivaceus) interleukin 12 (IL-12) -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences -
dc.relation.journalResearchArea Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor IL-12p35 -
dc.subject.keywordAuthor IL-12p40 -
dc.subject.keywordAuthor Single chain IL-12 -
dc.subject.keywordAuthor Functional IL-12 -
dc.subject.keywordAuthor Olive flounder -
dc.subject.keywordPlus INTERFERON-GAMMA -
dc.subject.keywordPlus INNATE RESISTANCE -
dc.subject.keywordPlus CYTOKINE -
dc.subject.keywordPlus IDENTIFICATION -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus MODULATION -
dc.subject.keywordPlus GENERATION -
dc.subject.keywordPlus P35 -

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