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MitchellRobertJames

Mitchell, Robert J.
Applied and Environmental Microbiology Lab.
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dc.citation.endPage 2923 -
dc.citation.number 17 -
dc.citation.startPage 2916 -
dc.citation.title LAB ON A CHIP -
dc.citation.volume 11 -
dc.contributor.author Park, Seongyong -
dc.contributor.author Kim, Dasol -
dc.contributor.author Mitchell, Robert J. -
dc.contributor.author Kim, Taesung -
dc.date.accessioned 2023-12-22T06:06:14Z -
dc.date.available 2023-12-22T06:06:14Z -
dc.date.created 2013-06-05 -
dc.date.issued 2011-09 -
dc.description.abstract We present a microfabricated concentrator array device that makes it possible to quantify the predation rate of Bdellovibrio bacteriovorus, a predatory microbe, toward its prey, Escherichia coli str. MG1655. The device can accumulate both prey and predator microbes sequentially within a series of concentrator arrays using the motility of the microbes and microfabricated arrowhead-shaped ratchet structures. Since the device can constrain both prey and predator cells within 200 pL chambers at a desired range of cell densities, it was demonstrated that the device cannot only enhance the possibility of studying predation processes/cycles directly at a single cell level but can also quantify the predation rates indirectly by measuring the time-dependent fluorescent intensity signals from the prey. Furthermore, the device can produce a wide range of initial prey to predator density ratios within various concentrator arrays through the use of microfluidic mixer structures on a single array chip, which allows us to study many different conditions with a single set of cultures, and quantitatively characterize the predation behaviour/rate. Lastly, we note that this novel concentrator array device can be a very powerful tool facilitating studies of microbial predations and microbe-microbe interaction and may be broadly used in other microbial biotechnological applications. -
dc.identifier.bibliographicCitation LAB ON A CHIP, v.11, no.17, pp.2916 - 2923 -
dc.identifier.doi 10.1039/c1lc20230h -
dc.identifier.issn 1473-0197 -
dc.identifier.scopusid 2-s2.0-80051647407 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/2745 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80051647407 -
dc.identifier.wosid 000293651100015 -
dc.language 영어 -
dc.publisher ROYAL SOC CHEMISTRY -
dc.title A microfluidic concentrator array for quantitative predation assays of predatory microbes -
dc.type Article -
dc.relation.journalWebOfScienceCategory Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology -
dc.relation.journalResearchArea Biochemistry & Molecular Biology; Chemistry; Science & Technology - Other Topics -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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