A microfluidic concentrator array for quantitative predation assays of predatory microbes
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- A microfluidic concentrator array for quantitative predation assays of predatory microbes
- Park, Seongyong; Kim, Dasol; Mitchell, Robert J.; Kim, Taesung
- BDELLOVIBRIO-BACTERIOVORUS; ESCHERICHIA-COLI; BACTERIAL CHEMOTAXIS; SURVIVAL STRATEGY; SWIMMING BACTERIA; GENE-EXPRESSION; HOST BACTERIA; CELLS; GRADIENTS; MICROBIOLOGY
- Issue Date
- ROYAL SOC CHEMISTRY
- LAB ON A CHIP, v.11, no.17, pp.2916 - 2923
- We present a microfabricated concentrator array device that makes it possible to quantify the predation rate of Bdellovibrio bacteriovorus, a predatory microbe, toward its prey, Escherichia coli str. MG1655. The device can accumulate both prey and predator microbes sequentially within a series of concentrator arrays using the motility of the microbes and microfabricated arrowhead-shaped ratchet structures. Since the device can constrain both prey and predator cells within 200 pL chambers at a desired range of cell densities, it was demonstrated that the device cannot only enhance the possibility of studying predation processes/cycles directly at a single cell level but can also quantify the predation rates indirectly by measuring the time-dependent fluorescent intensity signals from the prey. Furthermore, the device can produce a wide range of initial prey to predator density ratios within various concentrator arrays through the use of microfluidic mixer structures on a single array chip, which allows us to study many different conditions with a single set of cultures, and quantitatively characterize the predation behaviour/rate. Lastly, we note that this novel concentrator array device can be a very powerful tool facilitating studies of microbial predations and microbe-microbe interaction and may be broadly used in other microbial biotechnological applications.
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