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DC Field | Value | Language |
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dc.citation.endPage | 14361 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 14354 | - |
dc.citation.title | ACS APPLIED MATERIALS & INTERFACES | - |
dc.citation.volume | 11 | - |
dc.contributor.author | Kim, Soohyun | - |
dc.contributor.author | Kim, Hyunji | - |
dc.contributor.author | Qiao, Tian | - |
dc.contributor.author | Cha, Chaenyung | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Ro, Hyun Ji | - |
dc.contributor.author | Kim, Youngkyun | - |
dc.contributor.author | Lee, Wonmok | - |
dc.contributor.author | Lee, Hyunjung | - |
dc.date.accessioned | 2023-12-21T19:15:13Z | - |
dc.date.available | 2023-12-21T19:15:13Z | - |
dc.date.created | 2019-04-08 | - |
dc.date.issued | 2019-04 | - |
dc.description.abstract | For the safety of both production and life, it is a very significant issue to detect explosive nitro compounds in a remote way or over a long distance. Here, we report that nitro compounds were detected by the bacterial sensor based on hydrogel microbeads as a platform. Green fluorescent protein-producing Escherichia coli, which was genetically engineered to be sensitive to nitro compounds, was loaded within poly(2-hydroxyethyl methacrylate) [poly(HEMA)]-based hydrogel beads, in which fluorescent signals from bacteria were concentrated and strong enough to be easily detected. For efficient loading of negatively charged bacteria, the surface charge of poly(HEMA)-based beads was controlled by copolymerization with 2-(methacryloyloxy)-ethyltrimethylammonium chloride (MAETC) as a cationic monomer. With the addition of MAETC, the cell affinity was nine times enhanced by the interaction between the positively charged poly(HEMA-co-MAETC) beads and negatively charged bacteria. The increased cell affinity resulted in an enhancement of a sensing signal. After exposure to 2,4,6-trinitrotoluene, a typical explosive nitro compound, the fluorescence intensity of bacterial sensors using poly(HEMA-co-MAETC) beads having 80 wt % MAETC was five times increased compared to those based on poly(HEMA) beads. This amplification of the fluorescent signal enables easier detection of explosives efficiently by a remote detection, even over a long distance. | - |
dc.identifier.bibliographicCitation | ACS APPLIED MATERIALS & INTERFACES, v.11, no.15, pp.14354 - 14361 | - |
dc.identifier.doi | 10.1021/acsami.9b02262 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.scopusid | 2-s2.0-85064385908 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/26635 | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsami.9b02262 | - |
dc.identifier.wosid | 000465189000060 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Fluorescence Enhancement from Nitro-Compound-Sensitive Bacteria within Spherical Hydrogel Scaffolds | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology; Materials Science, Multidisciplinary | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics; Materials Science | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | poly(HEMA-co-MAETC) beads | - |
dc.subject.keywordAuthor | surface-charge-controlled hydrogel beads | - |
dc.subject.keywordAuthor | electrospraying method | - |
dc.subject.keywordAuthor | photopolymerization | - |
dc.subject.keywordAuthor | biosensor for TNT sensing | - |
dc.subject.keywordPlus | CONCANAVALIN-A | - |
dc.subject.keywordPlus | PHOTOPOLYMERIZATION | - |
dc.subject.keywordPlus | MICROSPHERES | - |
dc.subject.keywordPlus | ELECTROSPRAY | - |
dc.subject.keywordPlus | MICROBEADS | - |
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