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Author

Lee, Sung Kuk
Synthetic Biology Laboratory
Research Interests
  • - Design and optimization of metabolic pathways for value-added products

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Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels

Cited 8 times inthomson ciCited 6 times inthomson ci
Title
Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels
Author
Jung, Sang-KyuParisutham, VinuselviJeong, Seong HunLee, Sung Kuk
Keywords
ENGINEERED ESCHERICHIA-COLI; ZYMOMONAS-MOBILIS; BETA-GLUCOSIDASE; CLOSTRIDIUM-CELLULOVORANS; LIGNOCELLULOSIC BIOMASS; ETHANOL-PRODUCTION; SYNTHETIC BIOLOGY; CELLULASE GENE; ENDOGLUCANASE GENE; FERMENTABLE SUGARS
Issue Date
201209
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, v.2012, no., pp.1 - 10
Abstract
A major technical challenge in the cost-effective production of cellulosic biofuel is the need to lower the cost of plant cell wall degrading enzymes (PCDE), which is required for the production of sugars from biomass. Several competitive, low-cost technologies have been developed to produce PCDE in different host organisms such as Escherichia coli, Zymomonas mobilis, and plant. Selection of an ideal host organism is very important, because each host organism has its own unique features. Synthetic biology-aided tools enable heterologous expression of PCDE in recombinant E. coli or Z. mobilis and allow successful consolidated bioprocessing (CBP) in these microorganisms. In-planta expression provides an opportunity to simplify the process of enzyme production and plant biomass processing and leads to self-deconstruction of plant cell walls. Although the future of currently available technologies is difficult to predict, a complete and viable platform will most likely be available through the integration of the existing approaches with the development of breakthrough technologies.
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DOI
http://dx.doi.org/10.1155/2012/405842
ISSN
1110-7243
Appears in Collections:
ECHE_Journal Papers
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