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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 258 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 250 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 23 | - |
dc.contributor.author | Lee, Jae Hyeon | - |
dc.contributor.author | Lama, Suman | - |
dc.contributor.author | Kim, Jung Rae | - |
dc.contributor.author | Park, Sung Hoon | - |
dc.date.accessioned | 2023-12-21T21:07:01Z | - |
dc.date.available | 2023-12-21T21:07:01Z | - |
dc.date.created | 2018-06-09 | - |
dc.date.issued | 2018-03 | - |
dc.description.abstract | A range of recombinant strains of Escherichia coli were developed to produce 1,3-propanediol (1,3-PDO), an important C3 diol, from glucose. Two modules, the glycerol-producing pathway converting dihydroxyacetone phosphate to glycerol and the 1,3-PDO-producing pathway converting glycerol to 1,3-PDO, were introduced into E. coli. In addition, to avoid oxidative assimilation of the produced glycerol, glycerol oxidative pathway was deleted. Furthermore, to enhance the carbon flow to the Embden- Meyerhof-Parnas pathway, the Entner-Doudoroff pathway was disrupted by deleting 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Finally, the acetate production pathway was removed to minimize the production of acetate, a major and toxic by-product. Flask experiments were carried out to examine the performance of the developed recombinant E. coli. The best strain could produce 1,3-PDO with a yield of 0.47 mol/mol glucose. Along with 1,3-PDO, glycerol was produced with a yield of 0.33 mol/mol glucose. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.23, no.2, pp.250 - 258 | - |
dc.identifier.doi | 10.1007/s12257-018-0017-y | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-85046847171 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25406 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12257-018-0017-y | - |
dc.identifier.wosid | 000432151200016 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Production of 1,3-Propanediol from Glucose by Recombinant Escherichia coli BL21(DE3) | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.identifier.kciid | ART002345540 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Escherichia coli BL21(DE3) | - |
dc.subject.keywordAuthor | glycerol | - |
dc.subject.keywordAuthor | 1,3-PDO | - |
dc.subject.keywordAuthor | pathway engineering | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE J2B | - |
dc.subject.keywordPlus | 3-HYDROXYPROPIONIC ACID | - |
dc.subject.keywordPlus | CLOSTRIDIUM-BUTYRICUM | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | TRANSPORT | - |
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