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Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 572 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 564 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 23 | - |
dc.contributor.author | Nam Hoai Nguyen | - |
dc.contributor.author | Kim, Jung-Rae | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T20:12:18Z | - |
dc.date.available | 2023-12-21T20:12:18Z | - |
dc.date.created | 2018-11-29 | - |
dc.date.issued | 2018-09 | - |
dc.description.abstract | Application of the recently developed wholecell 3-HP biosensor, which detects 3-hydroxypropionic acid (3-HP) and expresses fluorescence as an output signal in response to the 3-HP concentration, was studied in three areas of enzyme and metabolic engineering. First, a sensor was applied to identify active 3-hydroxyaldehyde dehydrogenase (ALDH), a key enzyme in the 3-HP production pathway. Second, with the aid of helper enzymes that catalyze the conversion of 1,3-propanediol (1,3-PDO) to 3-HP, a 3-HP biosensor was converted into a 1,3-PDO biosensor. Third, a 3-HP biosensor, with proper modifications in expression module of the output signal and the use of pH-tolerant red fluorescent protein (RFP), was shown to monitor the rate of 3-HP production under process conditions in which one or more interfering compounds are present in the culture medium and/or the medium pH decreases. This study demonstrates that 3-HP biosensors can be widely used in enzyme and metabolic engineering applications for 3-HP production. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.23, no.5, pp.564 - 572 | - |
dc.identifier.doi | 10.1007/s12257-018-0390-6 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-85056329276 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25402 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12257-018-0390-6 | - |
dc.identifier.wosid | 000449943200010 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Application of Transcription Factor-based 3-Hydroxypropionic Acid Biosensor | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | 3-hydroxypropionic acid | - |
dc.subject.keywordAuthor | 1 | - |
dc.subject.keywordAuthor | 3-propanediol | - |
dc.subject.keywordAuthor | high-throughput screening | - |
dc.subject.keywordAuthor | Pseudomonas denitrificans | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | TOOLBOX | - |
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