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- Park, Sunghoon
- Biochemical Engineering Lab.
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Biocatalytic preparation of chiral epichlorohydrins using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis
- Author(s)
- Kim, Hee Sook, Lee, Jae-Hwa, Park, Sunghoon, Lee, Eun Yeol
- Issued Date
- 2004-01
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.9, no.1, pp.62 - 64
- Abstract
- The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinant Pichia pastoris with the enantioselective epoxide hydrolase of Rhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100% ee and a yield of 26% were obtained within 5 min from 50 mM racemates.
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- ISSN
- 1226-8372
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