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Author

Lee, Chang Young
Carbon Nanomaterials Lab
Research Interests
  • Carbon nanomaterials, gas sensors, neuroanalytical chemistry, biomimetic nanosystems

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Aptamer-functionalized nano-pattern based on carbon nanotube for sensitive, selective protein detection

Cited 2 times inthomson ciCited 2 times inthomson ci
Title
Aptamer-functionalized nano-pattern based on carbon nanotube for sensitive, selective protein detection
Author
Nam, KihwanEom, KilhoYang, JaemoonPark, JinsungLee, GyudoJang, KuewhanLee, HyungbeenLee, Sang WooYoon, Dae SungLee, Chang YoungKwon, Taeyun
Keywords
Aligned carbon nanotubes; Aptamers; Binding affinities; Biomolecular interactions; Carbon nanotube sensors; Detection sensitivity; Detection system; Drug screening; Effect of chemicals; Functionalized; Future applications; High sensitivity; Ion concentrations; Nano pattern; Protein detection; Quantitative characterization; Quantitative study; Specific detection
Issue Date
201211
Publisher
ROYAL SOC CHEMISTRY
Citation
JOURNAL OF MATERIALS CHEMISTRY, v.22, no.44, pp.23348 - 23356
Abstract
We have developed a horizontally aligned carbon nanotube sensor that enables not only the specific detection of biomolecules with ultra-sensitivity, but also the quantitative characterization of binding affinity between biomolecules and/or interaction between a carbon nanotube and a biomolecule, for future applications in early diagnostics. In particular, we have fabricated horizontally aligned carbon nanotubes, which were functionalized with specific aptamers that are able to specifically bind to biomolecules (i.e. thrombin). Our detection system is based on scanning probe microscopy (SPM) imaging for horizontally aligned aptamer-conjugated carbon nanotubes (ACNTs) that specifically react with target biomolecules at an ultra-low concentration. It is shown that the binding affinity between thrombin molecule and ACNT can be quantitatively characterized using SPM imaging. It is also found that the smart carbon nanotube sensor coupled with SPM imaging permits us to achieve the high detection sensitivity even up to similar to 1 pM, which is much higher than that of other bioassay methods. Moreover, we have shown that our method enables a quantitative study on small molecule-mediated inhibition of specific biomolecular interactions. In addition, we have shown that our ACNT-based system allows for the quantitative study of the effect of chemical environment (e.g. pH and ion concentration) on the binding affinity. Our study sheds light on carbon nanotube sensor coupled with SPM imaging, which opens a new avenue to early diagnostics and drug screening with high sensitivity.
URI
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DOI
http://dx.doi.org/10.1039/c2jm33688j
ISSN
0959-9428
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