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박성훈

Park, Sunghoon
Biochemical Engineering Lab.
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Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol

Author(s)
Yoo, Seung SikPark, SunghoonLee, Eun Yeol
Issued Date
2008-10
DOI
10.1007/s10529-008-9762-x
URI
https://scholarworks.unist.ac.kr/handle/201301/25358
Fulltext
https://link.springer.com/article/10.1007%2Fs10529-008-9762-x
Citation
BIOTECHNOLOGY LETTERS, v.30, no.10, pp.1807 - 1810
Abstract
The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatalyst. Enantiopure (S)-styrene oxide with 98% ee was obtained with 41% yield (maximum yield = 50%) from 1.8 M racemic styrene oxide at pH 8.0, 4 degrees C in the presence of 40% (v/v) Tween 20 and 5% (v/v) glycerol.
Publisher
SPRINGER
ISSN
0141-5492
Keyword (Author)
enantioselective hydrolysisepoxide hydrolaseglycerolsurfactant
Keyword
CATALYZED RESOLUTIONTRANSFORMATIONSSYSTEM

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