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DC Field | Value | Language |
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dc.citation.endPage | 5175 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 5169 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | - |
dc.citation.volume | 33 | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Kim, Seohyoung | - |
dc.contributor.author | Raj, S. Mohan | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T08:36:18Z | - |
dc.date.available | 2023-12-22T08:36:18Z | - |
dc.date.created | 2017-03-04 | - |
dc.date.issued | 2008-10 | - |
dc.description.abstract | Non-growing cells can function as whole-cell biocatalysts for hydrogen (H-2) production, a process that has recently drawn much attention. In order to evaluate their potential as whole-cell biocatalysts, we compared the H-2-production capability of four Enterobacteriaceae strains (Citrobacter amalonaticus Y19, Escherichia coli K-12 MG1655, Escherichia coli DJT135, and Enterobacter aerogenes) under growing and non-growing conditions. We evaluated their H-2-production activity at varying temperatures (25-45 degrees C) and pH conditions (6.0-8.0) using glucose or formate as the carbon source. Under growing conditions with 10 mM glucose as a substrate, E. aerogenes exhibited the highest H-2-production activity (17.0 +/- 0.2 mu mol H-2 mg cell (1) h(-1)) among the four strains, but the final H-2 yield was similar (1.7-1.8 mol H-2 mol(-1) glucose) in all four strains. H-2 production in the four strains proceeded through a formate-dependent pathway that involved the formate hydrogen lyase (FHL) complex. Under non-growing conditions with 20 mM formate as a substrate, we obtained high H-2-production activities, in the range of 95.5-195.2 mu mol H-2 mg cell(-1) h (1), with E. coli DJT135 exhibiting the highest activity (195.2 mu mol H-2 mg(-1) h(-1)) at pH 6.0 and 45 degrees C. In contrast, using glucose as the carbon substrate in non-growing cell experiments greatly reduced the H-2-production activity to 6.1-7.7 mu mol H-2 mg cell (1) h(-1). This study indicated that formate is a better substrate than glucose for H-2 production by lion-growing cells, and that the H2-production performance among the strains did not vary significantly, with the exception of E. coli K-12 MG1655. (C) 2008 International Association for Hydrogen Energy. | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.33, no.19, pp.5169 - 5175 | - |
dc.identifier.doi | 10.1016/j.ijhydene.2008.05.007 | - |
dc.identifier.issn | 0360-3199 | - |
dc.identifier.scopusid | 2-s2.0-52049115388 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25357 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0360319908006885 | - |
dc.identifier.wosid | 000260755600031 | - |
dc.language | 영어 | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Comparison of hydrogen-production capability of four different Enterobacteriaceae strains under growing and non-growing conditions | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Hydrogen production | - |
dc.subject.keywordAuthor | Citrobacter amalonaticus Y19 | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | Enterobacter aerogenes | - |
dc.subject.keywordAuthor | Specific activity | - |
dc.subject.keywordAuthor | Growing and non-growing cells | - |
dc.subject.keywordAuthor | Whole-cell biocatalyst | - |
dc.subject.keywordPlus | BACTERIUM RHODOPSEUDOMONAS-CAPSULATA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI STRAINS | - |
dc.subject.keywordPlus | BIOHYDROGEN PRODUCTION | - |
dc.subject.keywordPlus | H-2 METABOLISM | - |
dc.subject.keywordPlus | PALUSTRIS P4 | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CULTURES | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | Y19 | - |
dc.subject.keywordPlus | PH | - |
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