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DC Field | Value | Language |
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dc.citation.endPage | 1446 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1440 | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 43 | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Jo, Ji-Elin | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-22T08:13:33Z | - |
dc.date.available | 2023-12-22T08:13:33Z | - |
dc.date.created | 2017-03-04 | - |
dc.date.issued | 2008-12 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP) is an important platform chemical from which several commodity and specialty chemicals can be generated. The present investigation focuses on the construction and evaluation of a recombinant strain Escherichia coli SH254 that produces 3-HP from glycerol. The strain was developed by cloning two genes, dhaB of Klebsiella pneumoniae DSM 2026 encoding glycerol dehydratase and aldH of E. coli K-12 MG1655 encoding aldehyde dehydrogenase, respectively. In vitro assays of crude enzyme extract of glycerol dehydratase (DhaB) showed 37.0 U mg(-1) protein on glycerol with coenzyme B(12), and partially purified aldehyde dehydrogenase (AldH) exhibited 22.8 U mg(-1) protein on 3-hydroxypropionaldehyde (3-HPA) with NAD(+) as a cofactor. When cultivated aerobically on a glycerol medium containing yeast extract, the recombinant E. coli SH254 produced 3-HP at a maximum of 6.5 mmol l(-1) (0.58 g l(-1)), The highest specific rate and yield of 3-HP production were estimated as 6.6 mmol g(-1) cdw h(-1) and 0.48 mol mol(-1) glycerol, respectively. Although not optimized extensively, this study is encouraging for further development of a bioprocess to produce 3-HP from glycerol. | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.43, no.12, pp.1440 - 1446 | - |
dc.identifier.doi | 10.1016/j.procbio.2008.04.027 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.scopusid | 2-s2.0-54949113283 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/25355 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1359511308001530 | - |
dc.identifier.wosid | 000261322400017 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Glycerol | - |
dc.subject.keywordAuthor | 3-Hydroxypropionaldehyde | - |
dc.subject.keywordAuthor | 3-Hydroxypropionic acid | - |
dc.subject.keywordAuthor | Recombinant E. coli | - |
dc.subject.keywordAuthor | Glycerol dehydratase | - |
dc.subject.keywordAuthor | Aldehyde dehydrogenase | - |
dc.subject.keywordPlus | BIODIESEL PRODUCTION | - |
dc.subject.keywordPlus | ADENOSYLCOBALAMIN | - |
dc.subject.keywordPlus | DEHYDRATASE | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | PROTEINS | - |
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