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Ryu, Ja-Hyoung
Supramolecular Nanomaterials Lab.
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Array-Based Protein Sensing Using an Aggregation-Induced Emission (AIE) Light-Up Probe

Author(s)
Choi, HuyeonKim, SangpilLee, SeunghoKim, ChaekyuRyu, Ja-Hyoung
Issued Date
2018-08
DOI
10.1021/acsomega.8b01269
URI
https://scholarworks.unist.ac.kr/handle/201301/25110
Fulltext
https://pubs.acs.org/doi/10.1021/acsomega.8b01269
Citation
ACS OMEGA, v.3, no.8, pp.9276 - 9281
Abstract
Protein detection and identification are important for the diagnosis of diseases; however, the development of facile sensing probes still remains challenging. Here, we present an array-based "turn on" protein-sensing platform capable of detecting and identifying proteins using aggregation-induced emission luminogens (AIEgens). The water-soluble AIEgens in which fluorescence was initially turned off showed strong fluorescence in the presence of nanomolar concentrations of proteins via restriction of the intramolecular rotation of the AIEgens. The binding affinities between the AIEgens and proteins were associated with various chemical functional groups on AIEgens, resulting in distinct fluorescent-signal outcomes for each protein. The combined fluorescence outputs provided sufficient information to detect and discriminate proteins of interest by linear discriminant analysis. Furthermore, the array-based sensor enabled classification of different concentrations of specific proteins. These results provide novel insight into the use of the AIEgens as a new type of sensing probe in array-based systems.
Publisher
AMER CHEMICAL SOC
ISSN
2470-1343
Keyword
FLUORESCENT PROTEINIDENTIFICATIONNANOPARTICLESRECOGNITIONRECEPTORS

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