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AmblardFrancois

Amblard, Francois
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Deep line-temporal focusing with high axial resolution and a large field-of-view using intracavity control and incoherent pulse shaping

Author(s)
Lou, KaiWang, BoJee, Ah-YoungGranick, SteveAmblard, Francois
Issued Date
2018-10
DOI
10.1364/OL.43.004919
URI
https://scholarworks.unist.ac.kr/handle/201301/25083
Fulltext
https://www.osapublishing.org/ol/abstract.cfm?uri=ol-43-20-4919
Citation
OPTICS LETTERS, v.43, no.20, pp.4919 - 4922
Abstract
Line-temporal focusing has been recognized as an elegant strategy that provides two-photon microscopy with an effective means for fast imaging through parallelization, together with an improved resilience to scattering for deep imaging. However, the axial resolution remains sub-optimal, except when using high NA objectives and a small field-of-view. With the introduction of an intracavity control of the spectral width of the femtosecond laser to adaptively fill the back aperture of the objective lens, line-temporal focusing two-photon microscopy is demonstrated to reach near-diffraction-limited axial resolution with a large back-aperture objective lens, and improved immunity to sample scattering. In addition, a new incoherent flattop beam shaping method is proposed which provides a uniform contrast with little degradation of the axial resolution along the focus line, even deep in the sample. This is demonstrated in large volumetric imaging of mouse lung samples.
Publisher
OPTICAL SOC AMER
ISSN
0146-9592
Keyword
MULTIPHOTON MICROSCOPY2-PHOTON MICROSCOPYNEURONAL-ACTIVITYSCATTERING MEDIAILLUMINATIONTRANSPARENTBRAINLIGHT

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