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DC Field | Value | Language |
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dc.citation.endPage | 43 | - |
dc.citation.startPage | 36 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 126 | - |
dc.contributor.author | Bhardwaj, Jyoti | - |
dc.contributor.author | Sharma, Abhinav | - |
dc.contributor.author | Jang, Jaesung | - |
dc.date.accessioned | 2023-12-21T19:39:26Z | - |
dc.date.available | 2023-12-21T19:39:26Z | - |
dc.date.created | 2018-10-05 | - |
dc.date.issued | 2019-02 | - |
dc.description.abstract | We present a novel vertical flow-based paper immunosensor for the rapid and sensitive electrochemical and colorimetric detection of influenza H1N1 viruses using a different pore size (DP) sample pad. The DP sample pad consisted of two different pore size papers: larger pores (diameter: 11 mu m) facing the inlet, and smaller pores (diameter: 0.45 mu m) facing the conjugate pad. This sample pad offered moderate and uniform flows, and hence concentrated horseradish peroxidase tagged antibodies (HRP-Abs)-H1N1 complexes from 40 mu L of sample volumes on a conjugate pad for 2 min after sample injection, thereby providing fast detection (6 min for both detection methods) with 100 mu L of flushing afterwards, high sensitivity, and the simplicity of the sensor. The filtration characteristics of the DP sample pad were evaluated using fluorescent beads, indicating that only small sized bio-particles such as viruses can pass through the sample pad. Sandwich immunoreactions of HRP-Ab-H1N1-Ab were performed on the gold paper electrode of the immunoStrip, which was determined by electrochemical impedance spectroscopy (EIS) measurements. Simultaneously, the color signal of free HRP-Ab captured on the colorimetric zone was obtained using a scanner, and the intensity was analyzed using ImageJ. This immunosensor detected H1N1 virus concentration as low as 3.3 plaque forming units (PFU)/mL (phosphate buffer saline; PBS) and 4.7 PFU/mL (saliva) by EIS, and 1.34 PFU/mL (PBS) and 2.27 PFU/mL (saliva) by the colorimetric method. Furthermore, integrating these two detection methods can reduce false results with double assurance, and this device can provide a simple and economical on-site detection platform. | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.126, pp.36 - 43 | - |
dc.identifier.doi | 10.1016/j.bios.2018.10.008 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.scopusid | 2-s2.0-85055661681 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/24946 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0956566318308078?via%3Dihub | - |
dc.identifier.wosid | 000457659500006 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Vertical flow-based paper immunosensor for rapid electrochemical and colorimetric detection of influenza virus using a different pore size sample pad | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Vertical flow assay | - |
dc.subject.keywordAuthor | Electrochemical sensor | - |
dc.subject.keywordAuthor | Colorimetric sensor | - |
dc.subject.keywordAuthor | Sandwich immunoreaction | - |
dc.subject.keywordAuthor | H1N1 influenza virus | - |
dc.subject.keywordAuthor | Paper sensors | - |
dc.subject.keywordPlus | ANALYTICAL DEVICES | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | SENSOR | - |
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